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mouse anti shank3  (NeuroMab)


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    NeuroMab mouse anti shank3
    Mouse Anti Shank3, supplied by NeuroMab, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti shank3/product/NeuroMab
    Average 90 stars, based on 1 article reviews
    mouse anti shank3 - by Bioz Stars, 2026-06
    90/100 stars

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    Alterations in dendritic spine morphology in CA1 pyramidal neurons from R4ag11-Cre/DCC fl/fl mice. a Representative camera lucida reconstructions of CA1 pyramidal neuron apical dendrites from control (left) and R4ag11-Cre/DCC fl/fl mice. Scale bar = 1 μm. b Group data show no significant difference in average spine density between control and R4ag11-Cre/DCC fl/fl mice (left), but significant reductions in average spine length and spine head width (right). c Dendritic spine type classification reveals a significant increase in number of stubby-type spines (R4ag11-Cre/DCC fl/fl : 55.8 ± 4.6%, Control: 29.6 ± 0.9%; t 5.4 = 5.59, p = 0.002) and significant decrease in the number of mushroom-type dendritic spines (R4ag11-Cre/DCC fl/fl : 40.6 ± 4.9%, Control: 68.2 ± 1.0%; t 5.47 = 5.40, p = 0.002), with no detectable differences in thin-type spines ( p = 0.41). d - i Group data (top) and representative Western blots from hippocampal homogenates of control and R4ag11-Cre/DCC fl/fl mice showing levels of <t>SHANK1</t> ( d ), SHANK2 ( e ), SHANK3 ( f ), PSD-95 ( g ), p34-Arc ( h ), and phosphorylated S6 ribosomal protein ( i ). *: p < 0.05; **: p < 0.01. Data are presented as mean ± SEM
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    Alterations in dendritic spine morphology in CA1 pyramidal neurons from R4ag11-Cre/DCC fl/fl mice. a Representative camera lucida reconstructions of CA1 pyramidal neuron apical dendrites from control (left) and R4ag11-Cre/DCC fl/fl mice. Scale bar = 1 μm. b Group data show no significant difference in average spine density between control and R4ag11-Cre/DCC fl/fl mice (left), but significant reductions in average spine length and spine head width (right). c Dendritic spine type classification reveals a significant increase in number of stubby-type spines (R4ag11-Cre/DCC fl/fl : 55.8 ± 4.6%, Control: 29.6 ± 0.9%; t 5.4 = 5.59, p = 0.002) and significant decrease in the number of mushroom-type dendritic spines (R4ag11-Cre/DCC fl/fl : 40.6 ± 4.9%, Control: 68.2 ± 1.0%; t 5.47 = 5.40, p = 0.002), with no detectable differences in thin-type spines ( p = 0.41). d - i Group data (top) and representative Western blots from hippocampal homogenates of control and R4ag11-Cre/DCC fl/fl mice showing levels of <t>SHANK1</t> ( d ), SHANK2 ( e ), SHANK3 ( f ), PSD-95 ( g ), p34-Arc ( h ), and phosphorylated S6 ribosomal protein ( i ). *: p < 0.05; **: p < 0.01. Data are presented as mean ± SEM
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    Alterations in dendritic spine morphology in CA1 pyramidal neurons from R4ag11-Cre/DCC fl/fl mice. a Representative camera lucida reconstructions of CA1 pyramidal neuron apical dendrites from control (left) and R4ag11-Cre/DCC fl/fl mice. Scale bar = 1 μm. b Group data show no significant difference in average spine density between control and R4ag11-Cre/DCC fl/fl mice (left), but significant reductions in average spine length and spine head width (right). c Dendritic spine type classification reveals a significant increase in number of stubby-type spines (R4ag11-Cre/DCC fl/fl : 55.8 ± 4.6%, Control: 29.6 ± 0.9%; t 5.4 = 5.59, p = 0.002) and significant decrease in the number of mushroom-type dendritic spines (R4ag11-Cre/DCC fl/fl : 40.6 ± 4.9%, Control: 68.2 ± 1.0%; t 5.47 = 5.40, p = 0.002), with no detectable differences in thin-type spines ( p = 0.41). d - i Group data (top) and representative Western blots from hippocampal homogenates of control and R4ag11-Cre/DCC fl/fl mice showing levels of SHANK1 ( d ), SHANK2 ( e ), SHANK3 ( f ), PSD-95 ( g ), p34-Arc ( h ), and phosphorylated S6 ribosomal protein ( i ). *: p < 0.05; **: p < 0.01. Data are presented as mean ± SEM

    Journal: Molecular Brain

    Article Title: Pre- and post-synaptic roles for DCC in memory consolidation in the adult mouse hippocampus

    doi: 10.1186/s13041-020-00597-2

    Figure Lengend Snippet: Alterations in dendritic spine morphology in CA1 pyramidal neurons from R4ag11-Cre/DCC fl/fl mice. a Representative camera lucida reconstructions of CA1 pyramidal neuron apical dendrites from control (left) and R4ag11-Cre/DCC fl/fl mice. Scale bar = 1 μm. b Group data show no significant difference in average spine density between control and R4ag11-Cre/DCC fl/fl mice (left), but significant reductions in average spine length and spine head width (right). c Dendritic spine type classification reveals a significant increase in number of stubby-type spines (R4ag11-Cre/DCC fl/fl : 55.8 ± 4.6%, Control: 29.6 ± 0.9%; t 5.4 = 5.59, p = 0.002) and significant decrease in the number of mushroom-type dendritic spines (R4ag11-Cre/DCC fl/fl : 40.6 ± 4.9%, Control: 68.2 ± 1.0%; t 5.47 = 5.40, p = 0.002), with no detectable differences in thin-type spines ( p = 0.41). d - i Group data (top) and representative Western blots from hippocampal homogenates of control and R4ag11-Cre/DCC fl/fl mice showing levels of SHANK1 ( d ), SHANK2 ( e ), SHANK3 ( f ), PSD-95 ( g ), p34-Arc ( h ), and phosphorylated S6 ribosomal protein ( i ). *: p < 0.05; **: p < 0.01. Data are presented as mean ± SEM

    Article Snippet: The primary antibodies used were: goat polyclonal anti-DCC A20 (1:1000; Santa Cruz Biotechnology, RRID: AB_2245770), rabbit α-GAPDH (1:1000; Santa Cruz Biotechnology, RRID: AB_10167668), mouse anti-β-tubulin III (1:5000; Promega, RRID: AB_430874), rabbit α-phospho-S6 ribosomal protein (1:1000, Cell Signaling, RRID: AB_331682), mouse monoclonal α-PSD-95 (1:200, NeuroMAB, RRID: AB_2292909), mouse monoclonal α-Shank1 (1:200, NeuroMAB, RRID: AB_10673108), mouse monoclonal α-Shank2 (1:200, NeuroMAB, RRID: AB_2254586), mouse monoclonal α-Shank3 (1:200, NeuroMAB, RRID: AB_2187730), and rabbit α-p34-Arc/ARPC2 (1:1000, Millipore, RRID: AB_310447).

    Techniques: Western Blot